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The definition of menopausal status was based on the self-reported reproductive health questionnaire ( Females were defined as postmenopausal when they meet the following two criteria [32]: (1) firstly answered no to the question of Have you had at least one menstrual period in the past 12 months?, and (2) then answered hysterectomy or menopause/change of life to the question of What is the reason that you have not had a period in the past 12 months?

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The culture medium was then removed after 24 hours incubation

QIIME allows analysis of high-throughput community sequencing data